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KMID : 0613820080180040530
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Journal of Life Science
2008 Volume.18 No. 4 p.530 ~ p.536
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Effects of Pseudomonas fluorescens on Production of Several Inflammatory Mediators in the Human Alveolar Epithelial Cells
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Yang Hyun
Ryu Choong-Min
Park Seung-Hwan
Choi Hye-Jin
Kim Na-Yeon
Cho Hyung-Hoon
Ahn Jung-Hoon
Moon Yu-Seok
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Abstract
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To investigate the molecular mechanism of the airway inflammation by Pseudomonas fluorescens, effects on the inflammatory mediators such as interleukin-8 (IL-8), cyclooxygenase-2 (COX-2), macophage inhibitory cytokine 1 (MIC-1) were assessed in the human alveolar epithelial cells. Exposure to P. fluorescens and its recombinant bacteria suppressed cellular viability in the A549 epithelial cells and pro-inflammatory cytokine interleukin-8 production. However, pro-inflammatory prostaglandin-producing COX-2 protein was not altered by P. fluorescens though its mRNA was slightly elevated. As the inhibitory cytokine for the pro-inflammatory mediators, MIC-1 expression was monitored in A549 cells. MIC-1 gene induction was not significantly enhanced but the protein processing was changed by exposure to P. fluorescens. Pro-protein form of MIC-1 () was cleaved into active form mature MIC-1 () and propeptide () by the bacteria exposure. MIC-1 activation can contribute to the suppression of cellular viability by P. fluorescens and can retard IL-8-induced monocyte recruitment. However, sustained activation of MIC-1 can mediate the tissue injury by P. fluorescens exposure.
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KEYWORD
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Pseudomonas fluorescens, airway inflammation, macophage inhibitory cytokine 1, interleukin-8, cyclooxygenase-2
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